Mind Bomb 2/MIB2 Antibody

Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224] - Detection of Human MIB2/Skeletrophin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIB2/Skeletrophin antibody NB100-68224 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Mind Bomb 2/MIB2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 925 and 975 of human mindbomb homolog 2 (Skeletrophin) using the numbering given in entry NP_543151.1 (GeneID 142678).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MIB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:5000
• Application Notes: Immunoprecipitation Not recommended
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Mind Bomb 2/MIB2 Antibody

• E3 ubiquitin-protein ligase MIB2
• EC 6.3.2
• EC 6.3.2.-
• EC 6.3.2.13
• FLJ20648
• FLJ41406
• MIB2
• Mind Bomb 2
• Mind bomb homolog 2
• Mind Bomb-2
• mindbomb homolog 2 (Drosophila)
• Novel zinc finger protein
• Novelzin
• Putative NF-kappa-B-activating protein 002N
• SKD
• Skeletrophin
• Zinc finger ZZ type with ankyrin repeat domain protein 1
• ZZ type with ankyrin repeat domain 1
• ZZANK1
• ZZZ5

Background

MIB2/Skeletrophin is an actin-binding protein that bears two MIB/HERC2 domains, a ZZ-type zinc-finger domain, nine ankyrin repeats, and two RING-finger domains. The C-terminal RING domain has been found to have E3 ubiquitin ligase activity that mediates ubiquitination of the intracellular regions of the Notch receptor and its ligands to regulate Notch signaling. MIB2/Skeletrophin appears to be important to muscle development and may function as a suppressor of melanoma invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mind Bomb 2/MIB2 Antibody (NB100-68224)(2)

Publications using NB100-68224

• P P, S A, I S et al. TITLE: MIB2 variants altering NOTCH signaling result in left ventricle hypertrabeculation/non-compaction and are associated with Menetrier-like gastropathy. Human Molecular Genetics. 2017-07-25 [PMID: 28013292]
• Stempin CC, Chi L, Giraldo-Vela JP et al. The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation. J Biol Chem 2011-10-01 [PMID: 21896478]

Bioinformatics

• Gene Symbol: MIB2
• Entrez:
  • 142678(Human)
• Uniprot:
  • NP_543151.1(Human)