Mind Bomb 2/MIB2 Antibody

Images

 
Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224] - Detection of Human MIB2/Skeletrophin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224] - Detection of Human MIB2/Skeletrophin on HeLa whole cell lysate using NB100-68224. MIB2/Skeletrophin was also immunoprecipitated by another rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Mind Bomb 2/MIB2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 925 and 975 of human mindbomb homolog 2 (Skeletrophin) using the numbering given in entry NP_543151.1 (GeneID 142678).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MIB2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-68224.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mind Bomb 2/MIB2 Antibody

  • E3 ubiquitin-protein ligase MIB2
  • EC 6.3.2
  • EC 6.3.2.-
  • EC 6.3.2.13
  • FLJ20648
  • FLJ41406
  • MIB2
  • Mind Bomb 2
  • Mind bomb homolog 2
  • Mind Bomb-2
  • mindbomb homolog 2 (Drosophila)
  • Novel zinc finger protein
  • Novelzin
  • Putative NF-kappa-B-activating protein 002N
  • SKD
  • Skeletrophin
  • Zinc finger ZZ type with ankyrin repeat domain protein 1
  • ZZ type with ankyrin repeat domain 1
  • ZZANK1
  • ZZZ5

Background

MIB2/Skeletrophin is an actin-binding protein that bears two MIB/HERC2 domains, a ZZ-type zinc-finger domain, nine ankyrin repeats, and two RING-finger domains. The C-terminal RING domain has been found to have E3 ubiquitin ligase activity that mediates ubiquitination of the intracellular regions of the Notch receptor and its ligands to regulate Notch signaling. MIB2/Skeletrophin appears to be important to muscle development and may function as a suppressor of melanoma invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mind Bomb 2/MIB2 Antibody (NB100-68224)(2)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MIB2
Entrez
Uniprot