MIG2/Kindlin-2 Antibody (2G11) - Azide and BSA Free

Western Blot: MIG2/Kindlin-2 Antibody (2G11) [H00010979-M09] - Analysis of PLEKHC1 expression in transfected 293T cell line by PLEKHC1 monoclonal antibody (M09), clone 2G11. Lane 1: PLEKHC1 transfected lysatE (77.9 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MIG2/Kindlin-2 Antibody (2G11) [H00010979-M09] - Analysis of monoclonal antibody to FERMT2 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: MIG2/Kindlin-2 Antibody (2G11) [H00010979-M09] - Detection limit for recombinant GST tagged FERMT2 is 1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 2G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MIG2/Kindlin-2 Antibody (2G11) - Azide and BSA Free Summary

• Immunogen: PLEKHC1 (AAH17327, 1 a.a. ~ 680 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALDGIRMPDGCYADGTWELSVHVTDLNRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSSLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV
• Specificity: PLEKHC1 (2G11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FERMT2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein on ELISA. It has also been used for Western Blot.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MIG2/Kindlin-2 Antibody (2G11) - Azide and BSA Free

• fermitin family homolog 2 (Drosophila)
• fermitin family homolog 2
• fermitin family member 2
• FERMT2
• KIND2
• KIND2DKFZp686G11125
• kindlin 2
• Kindlin-2
• MIG2
• mig-2
• MIG2FLJ34213
• mitogen inducible gene 2 protein
• Mitogen-inducible gene 2 protein
• PH domain-containing family C member 1
• pleckstrin homology domain containing, family C (with FERM domain) member 1
• pleckstrin homology domain containing, family C member 1
• Pleckstrin homology domain-containing family C member 1
• PLEKHC1
• PLEKHC1FLJ44462
• UNC112
• UNC112B

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIG2/Kindlin-2 Antibody (H00010979-M09)(2)

Publications using H00010979-M09

• Dowling JJ, Vreede AP, Kim S et al. Kindlin-2 is required for myocyte elongation and is essential for myogenesis. BMC Cell Biol. 2008-07-08 [PMID: 18611274]
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: FERMT2
• Entrez:
  • 10979(Human)
• OMIM:
  • 607746(Human)
• Uniprot:
  • AAH17327(Human)