MICB Antibody [Unconjugated]

U21 expression destabilizes MICB.(A) MICB cells or MICB cells expressing U21 were pulse-labeled for 15 minutes and chased for 0, 2, or 6 hours. MICB (BMO2) and U21 were recovered from Triton X-100 lysates. Migration positions of MICB, U21, and class I MHC heavy chains are indicated, as are approximate molecular weight markers. Note that U21 and MICB migrate at nearly identical positions. (B) Quantification of (A) showing the percent MICB remaining after a 2 hr chase relative to the 0 hr chase point (n = 1). (C) The data represent the quantification of MICB stability in MICB or MICB-U21 cells from three independent experiments (n = 3). Error bars indicate the standard deviation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22102813), licensed under a CC-BY license. Not internally tested by R&D Systems.

U21 expression reduces steady state levels of mature and secreted MICB.(A) Immunoblot analysis of lysates or supernatants from MICB or MICB-U21 cells. Lysates and concentrated supernatants were prepared from 4×105 MICB or MICB-U21 cells. (B) MICB or MICB-U21 cells were pulse-labeled for 15 min and chased for 0, 0.5, 1, 2, and 4 hrs. MICB was recovered from Triton X-100 lysates (panel i) or cell supernatants (panel ii). Migration positions of MICB, soluble MICB (sMICB), and molecular weight markers are indicated. In U21-expressing cells, the faster-migrating mature (MICB*) and secreted (sMICB*) MICB are noted separately at the right. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22102813), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MICB Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MICB
  Ala23-Gly298
  Accession # CAI18747
• Specificity: Detects human MICB in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human MICA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MICB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICB Antibody [Unconjugated]

• MHC class I chain-related protein B
• MHC class I mic-B antigen
• MHC class I polypeptide-related sequence B
• MICB
• MIC-B
• PERB11.2MHC class I-like molecule PERB11.2-IMX
• stress inducible class I homolog

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Salih, H. et al. (2003) Blood 102:1389.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICB Antibody (AF1599)(6)

Publications using AF1599

• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (IHC, Human)
• Christine L. Schneider, Amy W. Hudson The Human Herpesvirus-7 (HHV-7) U21 Immunoevasin Subverts NK-Mediated Cytoxicity through Modulation of MICA and MICB PLoS Pathogens 11/10/2011 [PMID: 22102813]
• Sonia Agüera-González, Catharina C. Gross, Lola Fernández-Messina, Omodele Ashiru, Gloria Esteso, Howard C. Hang et al. Palmitoylation of MICA, a ligand for NKG2D, mediates its recruitment to membrane microdomains and promotes its shedding European Journal of Immunology 12/1/2011 [PMID: 21928280]
• Ohashi M, Eagle RA, Trowsdale J Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. J. Biol. Chem., 2010-03-19;285(22):16408-15. 2010-03-19 [PMID: 20304922] (Western Blot, Human)
• Wu JD, Atteridge CL, Wang X, Seya T, Plymate SR Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation. Clin. Cancer Res., 2009-01-15;15(2):632-40. 2009-01-15 [PMID: 19147769] (Western Blot, Mouse)
• Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2. Cancer Res., 2007-08-01;67(15):7458-66. 2007-08-01 [PMID: 17671216] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MICB
• Uniprot:
  • CAI18747()