MHC Class II RT1Bu Antibody (OX-3) [DyLight 755]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P
Clone
OX-3
Clonality
Monoclonal
Host
Mouse

MHC Class II RT1Bu Antibody (OX-3) [DyLight 755] Summary

Immunogen
Rat thymocyte membrane glycoproteins
Localization
Type I membrane protein. Localizes to late endocytic compartment. Associates with lysosome membranes.
Specificity
NB100-65011 recognizes a polymorphic determinant of rat Ia antigen (RT1Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing Ia positive cells from different rat strains, e.g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4). MRC OX-3 cross reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. NB100-65011 recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells (1,3,4). This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DRB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 755
Format
DyLight 755 (A=754, E=776)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Reactivity Notes

Reacts with Rat. Cross reacts with Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MHC Class II RT1Bu Antibody (OX-3) [DyLight 755]

  • Clone P2-beta-3
  • DR1
  • DR-1
  • DR12
  • DR-12
  • DR13
  • DR-13
  • DR14
  • DR-14
  • DR16
  • DR-16
  • DR4
  • DR-4
  • DR5
  • DR-5
  • DR7
  • DR-7
  • DR8
  • DR-8
  • DR9
  • DR-9
  • DRB1
  • DRw10
  • DRw11
  • DRw8
  • DW2.2/DR2.2
  • FLJ75017
  • FLJ76359
  • HLA class II antigen beta chain
  • HLA class II histocompatibility antigen, DR-1 beta chain
  • HLA-DR1B
  • HLA-DRB
  • HLA-DRB1*
  • HLA-DRB2
  • HLA-DR-beta 1
  • human leucocyte antigen DRB1
  • leucocyte antigen DR beta 1 chain
  • leucocyte antigen DRB1
  • lymphocyte antigen DRB1
  • major histocompatibility complex, class II, DR beta 1
  • MHC class II antigen DRB1*1
  • MHC class II antigen DRB1*10
  • MHC class II antigen DRB1*11
  • MHC class II antigen DRB1*12
  • MHC class II antigen DRB1*13
  • MHC class II antigen DRB1*14
  • MHC class II antigen DRB1*15
  • MHC class II antigen DRB1*16
  • MHC class II antigen DRB1*3
  • MHC class II antigen DRB1*4
  • MHC class II antigen DRB1*7
  • MHC class II antigen DRB1*8
  • MHC class II antigen DRB1*9
  • MHC class II antigen HLA-DR13
  • MHC class II HLA-DR beta 1 chain
  • MHC class II HLA-DR-beta cell surface glycoprotein
  • MHC class II HLA-DRw10-beta
  • SS1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-65011APC
DyLight 350 Labeled NB100-65011UV
DyLight 405 Labeled NB100-65011V
DyLight 405LS Labeled NB100-65011V3
DyLight 488 Labeled NB100-65011G
DyLight 680 Labeled NB100-65011FR
PE Labeled NB100-65011PE
PerCP Labeled NB100-65011PCP

Additional MHC Class II RT1Bu Products

MHC Class II RT1Bu NB100-65011IR

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol HLA-DRB1

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