MHC Class II Polymorphic Antibody (CI.13.I)

• Reactivity: Gp
• Applications: Flow, IHC
• Clone: CI.13.I
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MHC Class II Polymorphic Antibody (CI.13.I) Summary

• Immunogen: Guinea pig peritoneal T-cells
• Specificity: Reacts with the MHC Class II epitope present on strain 13, but not strain 2 guinea pig cells. The antigen is also found in outbred Hartley guinea pigs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRB1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant, 1% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MHC Class II Polymorphic Antibody (CI.13.I)

• Clone P2-beta-3
• DR1
• DR-1
• DR12
• DR-12
• DR13
• DR-13
• DR14
• DR-14
• DR16
• DR-16
• DR4
• DR-4
• DR5
• DR-5
• DR7
• DR-7
• DR8
• DR-8
• DR9
• DR-9
• DRB1
• DRw10
• DRw11
• DRw8
• DW2.2/DR2.2
• FLJ75017
• FLJ76359
• HLA class II antigen beta chain
• HLA class II histocompatibility antigen, DR-1 beta chain
• HLA-DR1B
• HLA-DRB
• HLA-DRB1*
• HLA-DRB2
• HLA-DR-beta 1
• human leucocyte antigen DRB1
• leucocyte antigen DR beta 1 chain
• leucocyte antigen DRB1
• lymphocyte antigen DRB1
• major histocompatibility complex, class II, DR beta 1
• MHC class II antigen DRB1*1
• MHC class II antigen DRB1*10
• MHC class II antigen DRB1*11
• MHC class II antigen DRB1*12
• MHC class II antigen DRB1*13
• MHC class II antigen DRB1*14
• MHC class II antigen DRB1*15
• MHC class II antigen DRB1*16
• MHC class II antigen DRB1*3
• MHC class II antigen DRB1*4
• MHC class II antigen DRB1*7
• MHC class II antigen DRB1*8
• MHC class II antigen DRB1*9
• MHC class II antigen HLA-DR13
• MHC class II HLA-DR beta 1 chain
• MHC class II HLA-DR-beta cell surface glycoprotein
• MHC class II HLA-DRw10-beta
• SS1

Background

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRB1