MHC Class I H-2Kb Antibody (AF6-88.5)

Product Discontinued

MHC Class I H-2Kb Antibody (AF6-88.5) Summary

• Immunogen: This antibody was generated by immunizing host with C57BL mouse splenocytes
• Localization: Nucleated cells of the H-2K^b haplotype
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2-K1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
• Application Notes: For immunofluorescent staining, the suggested use of this reagent is ≤0.25 ug per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse b haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MHC Class I H-2Kb Antibody (AF6-88.5)

• H2K
• H2Kb
• H-2Kb
• MHC class I H-2Kb

Background

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-K1