MHC Class I H-2Kb Antibody (AF6-88.5)

Product Discontinued

MHC Class I H-2Kb Antibody (AF6-88.5) Summary

• Immunogen: C57BL mouse splenocytes
• Specificity: Mouse b haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2-K1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.25 ug/10^6 cells in 100 ul
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application. IHC and IP use reported in literature.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for MHC Class I H-2Kb Antibody (AF6-88.5)

• H2K
• H2Kb
• H-2Kb
• MHC class I H-2Kb

Background

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-K1