METTL14 Antibody (CL4254) - BSA Free

Western Blot: METTL14 Antibody (CL4254) [NBP2-59044] - Analysis in human cell line A-431.

Immunocytochemistry/Immunofluorescence: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of RH-30 cells using the Anti-METTL14 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in the connective tissue.

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human endometrium shows nuclear immunoreactivity in glandular cells and in stroma.

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human testis shows nuclear positivity in cells in seminiferous tubules.

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human fallopian tube shows nuclear immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4254) [NBP2-59044] - Staining of human prostate shows strong nuclear positivity in glandular cells and in smooth muscle cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL4254
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

METTL14 Antibody (CL4254) - BSA Free Summary

• Description: Novus Biologicals Mouse METTL14 Antibody (CL4254) - BSA Free (NBP2-59044) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9HCE5, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: METTL14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for METTL14 Antibody (CL4254) - BSA Free

• EC 2.1.1
• EC 2.1.1.-
• KIAA1627
• KIAA1627methyltransferase-like protein 14
• methyltransferase like 14
• METTL14

Background

Probable methyltransferase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL14