METTL14 Antibody (CL4252) - Azide and BSA Free

Analysis in human testis and skeletal muscle tissues using NBP3-44125 antibody. Corresponding METTL14 RNA-seq data are presented for the same tissues.

Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.

Staining of human duodenum shows strong nuclear positivity in glandular cells.

Staining of human skeletal muscle shows only weak nuclear positivity in striated muscle fibers.

Analysis in human cell line A-431.

Staining of RH-30 cells using the Anti-METTL14 monoclonal antibody) .

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL4252
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

METTL14 Antibody (CL4252) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse METTL14 Antibody (CL4252) - Azide and BSA Free (NBP2-59043) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9HCE5, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: METTL14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for METTL14 Antibody (CL4252) - Azide and BSA Free

• EC 2.1.1
• EC 2.1.1.-
• KIAA1627
• KIAA1627methyltransferase-like protein 14
• methyltransferase like 14
• METTL14

Background

Probable methyltransferase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL14