Methyltransferase like 3 Antibody (1R6X6)

Immunoprecipitation: Methyltransferase like 3 Antibody (1R6X6) [Methyltransferase like 3] - Immunoprecipitation of Methyltransferase like 3 from 200 ug extracts of 293F cells was performed using 0.5 ug of Methyltransferase like 3 Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Methyltransferase like 3 Rabbit mAb at a dilution of 1:500.

Western Blot: Methyltransferase like 3 Antibody (1R6X6) [Methyltransferase like 3] - Western blot analysis of lysates from HeLa cells, using Methyltransferase like 3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 3min.

Immunocytochemistry/ Immunofluorescence: Methyltransferase like 3 Antibody (1R6X6) [Methyltransferase like 3] - Confocal imaging of HeLa cells using Methyltransferase like 3 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 1R6X6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Methyltransferase like 3 Antibody (1R6X6) Summary

• Description: Novus Biologicals Rabbit Methyltransferase like 3 Antibody (1R6X6) (NBP3-15652) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-250 of human Methyltransferase like 3 (Q86U44). SDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAMMGAVAEKKGPGEVAGTVTGQKRRAEQDSTTVAAFASSLVSGLNSSASEPAKEPAKKSRKHAASDVDLEIESLLNQQSTKEQQSKKVSQEILELL
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: METTL3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Methyltransferase like 3 Antibody (1R6X6)

• adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase
• EC 2.1.1.62
• IME4
• M6A
• methyltransferase like 3
• Methyltransferase-like protein 3
• MGC4336
• mRNA m(6)A methyltransferase
• MTA70
• MT-A70
• N6-adenosine-methyltransferase 70 kDa subunit
• Spo8

Background

Methyltransferase like 3 encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL3