MERIT40/HSPC142 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

MERIT40/HSPC142 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human MERIT40
  Asn182-Val329
  Accession # Q9NWV8
• Specificity: Detects human MERIT40 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MERIT40/HSPC142 Antibody [Alexa Fluor® 647]

• BABAM1
• BRISC and BRCA1 A complex member 1
• C10orf62
• C19orf62
• chromosome 19 open reading frame 62
• FLJ20571
• HSPC142
• mediator of Rap80 interactions and targeting 40 kDa
• Mediator of RAP80 interactions and targeting subunit of 40 kDa
• MERIT40
• MERIT40BRCA1-A complex subunit MERIT40
• NBA1
• NBA1BRISC and BRCA1 A complex member
• new component of the BRCA1 A complex
• New component of the BRCA1-A complex
• new component of the BRCAA1 A complex

Background

MERIT40 (MEdiator of Rap80 Interactions and Targeting subunit of 40 kDa; also NBA1 and c19orf62 and HSPC142) is a 40 kDa member of the MERIT40 family of proteins. It is ubiquitously expressed, and participates in the DNA damage response. At sites of double strand breaks, histones are phosphorylated, ubiquitinated, and deubiquitinated, with repairs performed by a sequentially formed complex that contains BRCA1, Rap80 and BRE. MERIT40 apparently serves as a scaffold protein that maintains the integrity of the complex. Human MERIT40 is 329 amino acids (aa) in length. It contains one VWF-A domain (aa 95-298) and six utilized Ser/Thr phosphorylation sites. There are three potential splice variants. One shows a 13 aa substitution for aa 234-329, a second possesses a 105 aa substitution for aa 263‑329, while a third shows a 37 aa substitution for aa 1-115, coupled to a deletion of aa 263-329. Over aa 182-329, human MERIT40 shares 95% aa identity with mouse MERIT40.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.