Melatonin R1A/MT1/MTNR1A Antibody

Product Discontinued

Melatonin R1A/MT1/MTNR1A Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human MTR1A. The immunogen region is located within amino acids 188 to 240.
• Specificity: MTR1A Antibody detects endogenous levels of total MTR1A protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTNR1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:500
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry-Frozen and western blot reported in scientific literature (PMID 24365461)
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Melatonin R1A/MT1/MTNR1A Antibody

• Mel1a receptor
• Mel-1A-R
• Melatonin R1A
• melatonin receptor 1A
• melatonin receptor type 1A
• MelatoninR1A
• MT1
• MTNR1A

Background

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Melatonin R1A/MT1/MTNR1A Antibody (NBP1-71113)(7)

Publications using NBP1-71113

• Kim MJ, Choi GE, Chae CW et al. Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR Cell death & disease 2023-02-21 [PMID: 36810730] (WB, ICC/IF, Human)
• Fan Y, Liang X, Wang R, Song L. Role of endogenous melatoninergic system in development of hyperalgesia and tolerance induced by chronic morphine administration in rats. Brain Res. Bull. 2017-10-06 [PMID: 28988976] (WB, Rat)
• Ryu Vitaly, Zarebidaki Eleen, Albers H Elliott et al. Short photoperiod reverses obesity in Siberian hamsters via sympathetically induced lipolysis and Browning in adipose tissue. Physiology & Behavior 2017-07-08 [PMID: 28694154] (IHC-FrFl, Hamster)
• Choi YJ, Habibi HR, Choi CY. Profiles of gonadotropin-inhibitory hormone and melatonin during the sex change and maturation of cinnamon clownfish, Amphiprion melanopus. Biochem. Biophys. Res. Commun. 2016-06-24 [PMID: 27208779] (IHC-P, Fish)
• Corthell JT, Olcese J, Trombley PQ. Melatonin in the mammalian olfactory bulb. Neuroscience 2013-12-21 [PMID: 24365461] (IHC-Fr, WB, Mouse)
• Fan Y, Liang X, Zheng B, Song L Melatonin Reduce Morphine-Induced Hyperalgesia and Tolerance of Rats via melatonin-MT1-PKC gamma Pathway. Research Square 2020-07-07
• Kim JS, Jung YH, Lee HJ et al. Melatonin activates ABCA1 via the BiP/NRF1 pathway to suppress high-cholesterol-induced apoptosis of mesenchymal stem cells Stem cell research & therapy 2021-02-05 [PMID: 33546749] (WB, Human)

FAQs for Melatonin R1A/MT1/MTNR1A Antibody (NBP1-71113). (Showing 1 - 2 of 2 FAQs).

1. I saw that you have the antibodies for the both receptors of melatonin but not listed with FACS application. I need more information about what is better for my assay.
   • At this time, none of our melatonin receptor antibodies have been tested and validated in Flow. Should you choose to purchase and test one of them, you would qualify for the Innovator's Rewards program. You should take a look at each individual antibody, the clonality, conjugates, immunogen region and all relevant data in order to decide which product would be most suitable for your needs.
2. I was wondering if you could give me some references of published article (specifically on rat tissue) that have used the product NBP1-7113 Melatonin receptor 1A antibody.
   • Unfortunately, we do not have any publications that utilize this antibody. I am sorry for any inconvenience. This product is guaranteed to detect the human, mouse, and rat proteins in ELISA and ICC. We have not yet tested this antibody for detection of the protein in tissue samples. Mouse and rat are listed based on homology and covered by the Novus Guarantee. I would also like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. Complete an online review with an image, detailing your positive or negative results of your study. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Alternatively, submit reviews information and image by emailing innovators@novusbio.com.

Bioinformatics

• Gene Symbol: MTNR1A
• Entrez:
  • 4543(Human)
  • 17773(Mouse)
  • 114211(Rat)
• OMIM:
  • 600665(Human)
• Uniprot:
  • P48039(Human)