Recombinant Human Melanocortin-1 R/MC1R GST (N-Term) Protein Summary
Description |
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 1-100 of Human Melanocortin-1 R/MC1R Source: Wheat Germ (in vitro) Amino Acid Sequence: MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLEVSISDGLFLSLGLVSLVENALVVATIAKNRNLHSPMYCFICCLALSDLLVSGSNVLETAVILL |
Preparation Method |
in vitro wheat germ expression system |
Details of Functionality |
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated. |
Source |
Wheat germ |
Protein/Peptide Type |
Recombinant Protein |
Gene |
MC1R |
Purity |
>80% by SDS-PAGE and Coomassie blue staining |
Applications/Dilutions
Dilutions |
- ELISA
- Immunoaffinity Purification
- Protein Array
- SDS-Page
- Western Blot
|
Theoretical MW |
36.74 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles. |
Buffer |
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer. |
Preservative |
No Preservative |
Purity |
>80% by SDS-PAGE and Coomassie blue staining |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human Melanocortin-1 R/MC1R GST (N-Term) Protein
Background
MC1R - melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for Melanocortin-1 R/MC1R Partial Recombinant Protein (H00004157-Q01). (Showing 1 - 1 of 1 FAQ).
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If I utilize the MC1 Receptor Partial Recombinant Protein (H00004157-Q01) you guys suggest as a positive control for your MC1R antibody on a Western blot, what amount should I use?
- You should load 0.1-0.2ug per lane for use in Western blot.
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