Melanocortin-1 R/MC1R Overexpression Lysate

Images

 
Western Blot: MC1 Receptor Overexpression Lysate (Adult Normal) [NBL1-12937] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for MC1 Receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Melanocortin-1 R/MC1R Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203218 and based on accession number NM_002386.
Epitope
C-terminal DDK tag
Specificity
Homo sapiens melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R), mRNA.
Gene
MC1R

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Reviewed Applications
Read 1 Review rated 5
using
NBL1-12937 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Melanocortin-1 R/MC1R Overexpression Lysate

  • CMM5
  • MC1R
  • MC1-R
  • melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
  • Melanocortin receptor 1
  • Melanocortin-1 R
  • Melanocortin1R
  • Melanocortin-1R
  • melanocyte-stimulating hormone receptor
  • melanotropin receptor
  • MSHR
  • MSH-R
  • Mshra
  • MSH-RMGC14337
  • SHEP2
  • Tob

Background

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Melanocortin-1 R/MC1R Lysate (NBL1-12937) (0)

There are no publications for Melanocortin-1 R/MC1R Lysate (NBL1-12937).
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Review for Melanocortin-1 R/MC1R Lysate (NBL1-12937) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBL1-12937:
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Western Blot Melanocortin-1 R/MC1R NBL1-12937
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reviewed by:
Anonymous
WB Human 11/19/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
Lot12937

Blocking

Blocking Details5% milk solution

Primary Anitbody

Dilution Ratio1:1000, 1 hour Incubation at RT

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP Conjugate
Secondary Concentration1:1000 in TBST for 1 hour

Details

Detection NotesVersadoc Densitometry at 30s

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Other Available Formats

NBL1-12937

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Recent Reviews

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1
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0
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2
0
1
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Anonymous
11/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MC1R