MEKK2 Antibody

Western Blot: MEKK2 Antibody [NBP2-24756] - Analysis of human MEKK2in human liver cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: MEKK2 Antibody [NBP2-24756] - Analysis of MEKK2 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

Product Discontinued

MEKK2 Antibody Summary

• Immunogen: A portion of amino acids 1-50 of human MEKK2 was used as the immunogen.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in Hamster, Mouse and Rat, and 82% homologous in Bovine and Equine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for MEKK2 Antibody

• EC 2.7.11.25
• MAP/ERK kinase kinase 2
• MAP3K2
• MAPK/ERK kinase kinase 2
• MAPKKK2
• MEK kinase 2
• MEKK 2
• MEKK2
• MEKK2B
• mitogen-activated protein kinase kinase kinase 2

Background

MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K2