MEKK2 Antibody (SC68-02)

Western Blot: MEKK2 Antibody (SC68-02) [NBP2-67615] - Analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Rat brain Lane 3: SW480

Immunocytochemistry/Immunofluorescence: MEKK2 Antibody (SC68-02) [NBP2-67615] - Staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEKK2 Antibody (SC68-02) [NBP2-67615] - Analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: MEKK2 Antibody (SC68-02) [NBP2-67615] - Analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MEKK2 Antibody (SC68-02) [NBP2-67615] - Staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MEKK2 Antibody (SC68-02) [NBP2-67615] - Staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEKK2 Antibody (SC68-02) [NBP2-67615] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

Product Discontinued

MEKK2 Antibody (SC68-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MEKK2 aa 1-47 / 619. (SwissProt: Q9Y2U5 Human; SwissProt: Q61083 Mouse; Unigene: 213212 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP3K2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MEKK2 Antibody (SC68-02)

• EC 2.7.11.25
• MAP/ERK kinase kinase 2
• MAP3K2
• MAPK/ERK kinase kinase 2
• MAPKKK2
• MEK kinase 2
• MEKK 2
• MEKK2
• MEKK2B
• mitogen-activated protein kinase kinase kinase 2

Background

MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K2