MEK5 Antibody (M1-E6) - Azide and BSA Free

Western Blot: MEK5 Antibody (M1-E6) [H00005607-M01] - Analysis of MAP2K5 expression in transfected 293T cell line by MAP2K5 monoclonal antibody (M01), clone M1-E6.Lane 1: MAP2K5 transfected lysate (Predicted MW: 50.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MEK5 Antibody (M1-E6) [H00005607-M01] - Analysis of monoclonal antibody to MAP2K5 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: MEK5 Antibody (M1-E6) [H00005607-M01] - MAP2K5 monoclonal antibody (M01), clone M1-E6 Analysis of MAP2K5 expression in Jurkat.

Western Blot: MEK5 Antibody (M1-E6) [H00005607-M01] - MAP2K5 monoclonal antibody (M01), clone M1-E6. Analysis of MAP2K5 expression in K-562.

Sandwich ELISA: MEK5 Antibody (M1-E6) [H00005607-M01] - Detection limit for recombinant GST tagged MAP2K5 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: M1-E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MEK5 Antibody (M1-E6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAP2K5 (AAH08838, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGESSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP
• Specificity: MAP2K5 - mitogen-activated protein kinase kinase 5
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MEK5 Antibody (M1-E6) - Azide and BSA Free

• HsT17454
• MAP kinase kinase 5
• MAP kinase kinase MEK5b
• MAP2K5
• MAPK/ERK kinase 5
• MAPKK 5
• MAPKK5
• MAPKK5EC 2.7.12.2
• MEK5
• MEK5MEK 5
• mitogen-activated protein kinase kinase 5
• MKK5
• PRKMK5
• PRKMK5dual specificity mitogen-activated protein kinase kinase 5

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K5
• Entrez:
  • 5607(Human)
• OMIM:
  • 602520(Human)
• Uniprot:
  • AAH08838(Human)