Human, Mouse, Rat MEK1 [p Ser222, p Ser218] ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat MEK1 [p Ser222, p Ser218] ELISA Kit (Colorimetric) [NBP2-62159] - A typical standard curve for MEK1 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Human, Mouse, Rat MEK1 [p Ser222, p Ser218] ELISA Kit (Colorimetric) Summary

• Modification: p Ser218, p Ser222
• Specificity: Crossreactivity: pMEK 1 (100%), pMEK 2 (12.2%) and <0.1%: MEK-1 (inactive), phospho Jnk, Erk2, phospho Erk2
• Standard Curve Range: 187.5 - 6,000 pg/ml
• Sensitivity: 85.2 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Low Concentration %CV - 14.8% Medium Concentration %CV - 7.9% High Concentration %CV - 3.9%
• Intra-Assay: Low Concentration %CV - 4.7% Medium Concentration %CV - 4.1% High Concentration %CV - 3.4%
• Spike Recovery: Jurkat cells 1 million cells/mL - 99.6%
  2 million cells/mL - 95.2%
  4 million cells/mL - 96.9%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MAP2K1

Applications/Dilutions

• Dilutions:
  • ELISA

Reactivity Notes

Crossreactivity: pMEK 1 (100%), pMEK 2 (12.2%) and <0.1%: MEK-1 (inactive), phospho Jnk, Erk2, phospho Erk2

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human, Mouse, Rat MEK1 [p Ser222, p Ser218] ELISA Kit (Colorimetric)

• dual specificity mitogen-activated protein kinase kinase 1
• ERK activator kinase 1
• MAP kinase kinase 1
• MAP2K1
• MAPK/ERK kinase 1
• MAPKK1
• MEK1
• MEK1MAPKK 1
• mitogen-activated protein kinase kinase 1
• MKK1
• MKK1MEK 1
• PRKMK1EC 2.7.12.2
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MEK1 ELISA Kit (NBP2-62159). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MAP2K1