MEK1 Antibody

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Western blot shows lysates of BT-20 human breast cancer cell line, U937 human histiocytic lymphoma cell line, DU145 human prostate carcinoma cell line, and mouse brain tissue. PVDF membrane was probed with 0.2 µg/mL ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MEK1 Antibody Summary

Immunogen
E. coli-derived recombinant human MEK1
Lys2-Val393 (Ser218Asp, Ser222Asp)
Accession # Q02750
Specificity
Detects human, mouse, and rat MEK1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAP2K1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEK1 Antibody

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP2K1
Uniprot