MEK1/2 [p Ser218, p Ser222, p Ser226, p Ser222, p Ser226] Antibody (1020E) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
1020E
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

MEK1/2 [p Ser218, p Ser222, p Ser226, p Ser222, p Ser226] Antibody (1020E) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing the human, mouse, and rat MEK1 S218/S222 site
Modification
p Ser222, p Ser226, p Ser218, p Ser222, p Ser226
Specificity
Detects human, mouse, and rat MEK1 and MEK2 dually phosphorylated at S218/S222 and S222/S226, respectively.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAP2K1
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEK1/2 [p Ser218, p Ser222, p Ser226, p Ser222, p Ser226] Antibody (1020E) [Unconjugated]

  • CFC3
  • CFC4
  • dual specificity mitogen-activated protein kinase kinase 1
  • dual specificity mitogen-activated protein kinase kinase 2
  • ERK activator kinase 1
  • ERK activator kinase 2
  • MAP kinase kinase 2
  • MAPK/ERK kinase 1
  • MAPK/ERK kinase 2
  • MAPKK 1
  • MAPKK1
  • MAPKK2
  • MEK 1
  • MEK1
  • MEK1/MEK2
  • MEK2
  • MEL
  • mitogen-activated protein kinase kinase 2, p45
  • MKK1
  • MKK2
  • PRKMK1
  • PRKMK2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks, including the Raf kinases. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAP2K1