HAF008). A specific band was detected for Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) Monoclonal Antibody (Catalog # MAB8407) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MEK1 (S218/S222)/ MEK2 (S222/S226) at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8407-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21632-50ul | |||
![Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] - Western blot analysis using NBP3-21632 in (1) 293T cell lysate; (2) A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21632_rabbit-mek1-2-mab-sr1751-672023106240.jpg "Western Blot: MEK1/2 Antibody (SR1751) [NBP3-21632] - Western blot analysis using NBP3-21632 in (1) 293T cell lysate; (2) A549 cell lysate.")
![Immunohistochemistry: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21632.](http://images.novusbio.com/fullsize/nbp3-21632_rabbit-mek1-2-mab-sr1751-57202316112133.jpg)
![Immunocytochemistry/Immunofluorescence: MEK1/2 Antibody (SR1751) [NBP3-21632] - Immunofluorescent analysis of Hela cells, using NBP3-21632.](http://images.novusbio.com/fullsize/nbp3-21632_rabbit-mek1-2-mab-sr1751-113202515145474.jpg)
![Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).](http://images.novusbio.com/fullsize/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-2711202419553094.jpg "Immunohistochemistry: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/2 (p Ser221, p Ser217).")
![Western Blot: MEK1/2 [p Ser221, p Ser217] Antibody - BSA Free [NBP3-20686] - Western blot analysis of lysed extracts from HeLa cell untreated or treated with UV MEK1/2 (p Ser221, p Ser217)](http://images.novusbio.com/fullsize/nbp3-20686_rabbit-mek1-2-p-ser217-p-ser221-pab-271120242074623.jpg)
![Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0002.jpg "Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of PC-12 cells, using MEK1/2 antibody (NBP3-05651) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MEK1-2-Antibody-Western-Blot-NBP3-05651-img0001.jpg)
