MED7 Antibody (PCRP-MED7-1B8) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-MED7-1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

MED7 Antibody (PCRP-MED7-1B8) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Recombinant full-length human MED7 protein (Uniprot: O43513)
• Localization: Nucleus, Nuclear bodies
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Protein Array
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MED7 Antibody (PCRP-MED7-1B8) [CoraFluor™ 1]

• Activator-recruited cofactor 34 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 9
• cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)
• cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
• CRSP33CRSP complex subunit 9
• CRSP9hMED7
• mediator complex subunit 7ARC34
• mediator of RNA polymerase II transcription subunit 7
• MGC12284
• RNA polymerase transcriptional regulation mediator subunit 7 homolog
• Transcriptional coactivator CRSP33

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED7