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MED4 Antibody Blocking Peptide

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC
Concentration
0.2 mg/ml

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MED4 Antibody Blocking Peptide Summary

Description
A blocking peptide containing 17 amino acids near the carboxy terminus of human MED4.

Source: Synthetic

(Accession #: NP_054885)
Source
Synthetic
Protein/Peptide Type
Antibody Blocking Peptide
Gene
MED4
Purity
N/A

Applications/Dilutions

Dilutions
  • Antibody Competition
Application Notes
This peptide is useful as a blocking peptide for NBP1-76930.

For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Purity
N/A

Alternate Names for MED4 Antibody Blocking Peptide

  • Activator-recruited cofactor 36 kDa component
  • ARC36
  • DRIP36
  • DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
  • FLJ10956
  • HSPC126
  • HSPC126vitamin D receptor interacting protein
  • MED4
  • mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
  • subunit 4 homolog
  • TRAP36
  • VDRIP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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AF5520
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
(2 Reviews)
Species: Hu, Mu
Applications: WB
LIGHT/TNFSF14
[Unconjugated]
664-LI/CF
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
(17 Publications)
Species: Hu
Applications: BA
VDR/NR1I1/Vitamin
D Receptor Antibody ( ...
NBP2-66778
Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
CYP27B1 Antibody
NBP2-92637
Western Blot: CYP27B1 Antibody [NBP2-92637] - Analysis of extracts of various cell lines, using CYP27B1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Species: Hu, Rt
Applications: ICC/IF, WB
MBD4 Antibody -
BSA Free
NBP1-91189
Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: MBD4 Antibody [NBP1-91189] - IHC analysis of MBD4 in human breast cancer cells using DAB with hematoxylin counterstain.
(1 Publication)
Species: Hu, Mu(-)
Applications: ICC/IF, IHC, IHC-P, WB (-)
MED10 Antibody
NBP3-13045
Western Blot: MED10 Antibody [NBP3-13045] - 293T whole cell and nuclear extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 antibody (NBP3-13045) diluted at 1:500.
Immunocytochemistry/Immunofluorescence: MED10 Antibody [NBP3-13045] - MED10 antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 antibody (NBP3-13045) diluted at 1:500. Blue: Hoechst 33342 staining.
Species: Hu
Applications: ICC/IF, WB
NCOR1 Antibody
NB100-58824
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
(3 Publications)
Species: Hu, Mu
Applications: CHIP-SEQ, ChIP, IP, WB
TRACP/PAP/ACP5
Antibody
H00000054-D01P
Western Blot: ACP5 Antibody [H00000054-D01P] - Analysis of ACP5 expression in transfected 293T cell line by ACP5 polyclonal antibody.Lane 1: ACP5 transfected lysate(36.60 KDa).Lane 2: Non-transfected lysate.
Western Blot: ACP5 Antibody [H00000054-D01P] - Analysis of ACP5 expression in human liver.
(3 Publications)
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Applications: IHC, IHC-P, WB
TDRD7 Antibody
NBP1-87315
Immunohistochemistry: TDRD7 Antibody [NBP1-87315] - Staining of human testis shows cytoplasmic and nuclear positivity in subsets of testicular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
MED21 Antibody
(3E9)
H00009412-M08
ELISA: MED21 Antibody (3E9) [H00009412-M08] - Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.
Immunocytochemistry/Immunofluorescence: MED21 Antibody (3E9) [H00009412-M08] - Analysis of monoclonal antibody to SURB7 on HeLa cell. Antibody concentration 10 ug/ml
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Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NCOA6 Antibody
NB200-335
Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(7 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, WB
CD40 Ligand/TNFSF5
[HRP]
DCDL40
(41 Publications)
Species: Hu
Applications: ELISA
SCYL1 Antibody
(2E5)
H00057410-M02
Sandwich ELISA: SCYL1 Antibody (2E5) [H00057410-M02] - Detection limit for recombinant GST tagged SCYL1 is 0.03 ng/ml as a capture antibody.
Western Blot: SCYL1 Antibody (2E5) [H00057410-M02] - SCYL1 monoclonal antibody (M02), clone 2E5. Analysis of SCYL1 expression in PC-12.
Western Blot: SCYL1 Antibody (2E5) [H00057410-M02] - SCYL1 monoclonal antibody (M02), clone 2E5. Analysis of SCYL1 expression in Hela S3 NE.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, S-ELISA, WB
MED8 Antibody
NBP1-89014
Western Blot: MED8 Antibody [NBP1-89014] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MED8 Antibody [NBP1-89014] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunocytochemistry/Immunofluorescence: MED8 Antibody [NBP1-89014] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MYBBP1A Antibody
NB100-61050
Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-MYBBP1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of human MYBBP1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MYBBP1A antibody NB100-61050 used for IP at 3 ug per reaction. MYBBP1A was also immunoprecipitated by rabbit anti-MYBBP1A antibody NB100-61051. For blotting immunoprecipitated MYBBP1A, NB100-61051 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, PLA, WB
TRAF-2 Antibody
NB100-56173
Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Citeab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY licence.
Immunohistochemistry: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human melanoma tissue section stained for TRAF2 at 1:2000. A1 is a higher magnification of A. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.
(3 Publications)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(15 Publications)
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MED7 Antibody (3E7)
H00009443-M01
Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi ( Cat # H00009443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRSP9 monoclonal antibody (M01), clone 3E7 (Cat # H00009443-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: MED7 Antibody (3E7) [H00009443-M01] - Detection limit for recombinant GST tagged CRSP9 is approximately 0.3ng/ml as a capture antibody.
Western Blot: MED7 Antibody (3E7) [H00009443-M01] - Analysis of CRSP9 expression in transfected 293T cell line by CRSP9 monoclonal antibody (M01), clone 3E7.Lane 1: CRSP9 transfected lysate(27.2 KDa).Lane 2: Non-transfected lysate.
Species: Hu
Applications: ELISA, KD, S-ELISA, WB
ARC/NOL3 Antibody
NBP2-19561
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NOL3 stained by NOL3 antibody diluted at 1:500.
Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Mouse brain. NOL3 stained by NOL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
MED4 Antibody
Blocking Peptide
NBP1-76930PEP
MED4 Antibody Blocking Peptide
Species: Hu
Applications: AC

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Bioinformatics

Gene Symbol MED4