![Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of TRFP expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MED20-Antibody-Western-Blot-H00009477-B01P-img0005.jpg "Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of TRFP expression in Hela S3 NE.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | MED20 (NP_004266.2, 1 a.a. - 212 a.a.) full-length human protein. MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR |
| Specificity | MED20 - mediator complex subunit 20, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Mouse |
| Gene | MED20 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It is also useful for immunofluoresence. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western ACE-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE2_AF933_Simple_Western_18669.jpg)
![Immunohistochemistry ACE-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE-2_AF933_Immunohistochemistry_9800.jpg)
![Immunohistochemistry ACE-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE-2_AF933_Immunohistochemistry_10025.jpg)
| Publication using H00009477-B01P | Applications | Species |
|---|---|---|
| Sato S, Tomomori-Sato C, Tsai KL et al. Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme. J Biol Chem 2016-12-23 [PMID: 27821593] |
Secondary Antibodies |
Isotype Controls |
Research Areas for MED20 Antibody (H00009477-B01P)Find related products by research area.
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| Gene Symbol | MED20 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: MED20 Antibody [H00009477-B01P] - Analysis of purified antibody to TRFP on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MED20-Antibody-Immunocytochemistry-Immunofluorescence-H00009477-B01P-img0004.jpg "Immunocytochemistry/Immunofluorescence: MED20 Antibody [H00009477-B01P] - Analysis of purified antibody to TRFP on HeLa cell. (antibody concentration 10 ug/ml)")
![Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of MED20 expression in transfected 293T cell line by MED20 polyclonal antibody. Lane 1: TRFP transfected lysate(23.32 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MED20-Antibody-Western-Blot-H00009477-B01P-img0006.jpg "Western Blot: MED20 Antibody [H00009477-B01P] - Analysis of MED20 expression in transfected 293T cell line by MED20 polyclonal antibody. Lane 1: TRFP transfected lysate(23.32 KDa). Lane 2: Non-transfected lysate.")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")