MDL-1/CLEC5A Antibody (226402) [Unconjugated]

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." > MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." title="RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A Monoclonal Antibody (MAB1639, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins." />

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A Monoclonal Antibody (MAB1639, filled histogram) or isotype control antibody (<a class=

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 226402
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

MDL-1/CLEC5A Antibody (226402) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A
• Specificity: Detects mouse MDL-1/CLEC5A. In ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CLEC5A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MDL-1/CLEC5A Antibody (226402) [Unconjugated]

• CLEC5A
• CLECSF5
• CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5
• C-type lectin domain family 5, member A
• C-type lectin superfamily member 5
• MDL1
• MDL-1
• MDL-1MGC138304
• Myeloid DAP12-associating lectin 1
• myeloid DAP12-associating lectin-1

Background

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MDL-1/CLEC5A Antibody (MAB1639)(3)

Publications using MAB1639

• BW Wortham, BL Eppert, JL Flury, SM Garcia, WR Donica, A Osterburg, B Joyce-Shai, DJ Cua, MT Borchers Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies J. Immunol., 2016-02-29;196(8):3227-31. 2016-02-29 [PMID: 26927798] (Flow Cytometry, IHC, IHC-P, Mouse)
• Charles , Julia F, Hsu , Lih-Yun, Niemi , Erene C, Weiss , Arthur, Aliprantis , Antonios, Nakamura , Mary C Inflammatory arthritis increases mouse osteoclast precursors with myeloid suppressor function. J Clin Invest, 2012-11-01;122(12):4592-605. 2012-11-01 [PMID: 23114597] (Flow Cytometry, Mouse)
• Ingersoll MA, Spanbroek R, Lottaz C Comparison of gene expression profiles between human and mouse monocyte subsets. Blood, 2009-11-12;115(3):e10-9. 2009-11-12 [PMID: 19965649] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CLEC5A