Max Overexpression Lysate

Images

 
Western Blot: MAX Overexpression Lysate (Adult Normal) [NBL1-12921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAX.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Max Overexpression Lysate Summary

Description
Transient overexpression lysate of MYC associated factor X (MAX), transcript variant 5 The lysate was created in HEK293T cells, using Plasmid ID RC206294 and based on accession number NM_145116. The protein contains a C-MYC/DDK Tag.
Gene
MAX

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
15.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Max Overexpression Lysate

  • BHLHD4
  • bHLHd4MGC11225
  • bHLHd5
  • bHLHd6
  • bHLHd7
  • bHLHd8
  • Class D basic helix-loop-helix protein 4
  • helix-loop-helix zipper protein
  • MAX protein
  • Max
  • MGC10775
  • MGC18164
  • MGC34679
  • MGC36767
  • MYC associated factor X
  • Myc-associated factor X
  • orf1
  • protein max

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-89979
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-24509
Species: Hu, Mu, Rt, Bv, Ca, Ma-Op, Pm
Applications: WB, IHC, IHC-P
NBP1-32775
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-04052
Species: Hu
Applications: WB, IP
NB600-799
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-88626
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-38290
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-32887
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-109
Species: Hu, Mu
Applications: WB, ChIP, Flow, GS, ICC/IF, IP, CyTOF-ready
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF3760
Species: Hu
Applications: WB
NBP2-46060
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Max Lysate (NBL1-12921) (0)

There are no publications for Max Lysate (NBL1-12921).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Max Lysate (NBL1-12921) (0)

There are no reviews for Max Lysate (NBL1-12921). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Max Lysate (NBL1-12921) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-12921

Additional Max Products

Bioinformatics Tool for Max Lysate (NBL1-12921)

Discover related pathways, diseases and genes to Max Lysate (NBL1-12921). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Max Lysate (NBL1-12921)

Discover more about diseases related to Max Lysate (NBL1-12921).
 

Pathways for Max Lysate (NBL1-12921)

View related products by pathway.

PTMs for Max Lysate (NBL1-12921)

Learn more about PTMs related to Max Lysate (NBL1-12921).
 

Research Areas for Max Lysate (NBL1-12921)

Find related products by research area.

Blogs on Max.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Max Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MAX