Max Antibody

Western Blot: Max Antibody [H00004149-D01P] - Analysis of MAX expression in transfected 293T cell line by MAX polyclonal antibody.Lane 1: MAX transfected lysate(17.20 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: Max Antibody [H00004149-D01P] - Analysis of protein-protein interactions between MAX and SMAD3. HeLa cells were stained with anti-MAX rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Max Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MAX (NP_660087.1, 1 a.a. - 151 a.a.) full-length human protein. MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS
• Specificity: MAX - MYC associated factor X,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Max Antibody

• BHLHD4
• bHLHd4MGC11225
• bHLHd5
• bHLHd6
• bHLHd7
• bHLHd8
• Class D basic helix-loop-helix protein 4
• helix-loop-helix zipper protein
• MAX protein
• Max
• MGC10775
• MGC18164
• MGC34679
• MGC36767
• MYC associated factor X
• Myc-associated factor X
• orf1
• protein max

Background

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Max Antibody (H00004149-D01P)(1)

Publication using H00004149-D01P

• Pichulik T, Khatamzas E, Liu X et al. Pattern recognition receptor mediated downregulation of microRNA-650 fine-tunes MxA expression in dendritic cells infected with Influenza A virus. Eur J Immunol. 2015-10-08 [PMID: 26460926]

Bioinformatics

• Gene Symbol: MAX
• Entrez:
  • 4149(Human)
• Uniprot:
  • NP_660087.1(Human)