MATN3 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MATN3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Matrilin-3
• Specificity: Detects human Matrilin-3 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Matrilin-3 is observed and less than 1% cross-reactivity with recombinant human (rh) Matrilin-2 and rhMatrilin-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MATN3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MATN3 Antibody [Unconjugated]

• DIPOA
• EDM5
• HOA
• matrilin 3
• Matrilin3
• Matrilin-3
• NDUFC2
• OADIP
• OS2

Background

Matrilin-3 is a 50-60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix (1-3). The human Matrilin-3 cDNA encodes a 486 amino acid (aa) precursor with a 28 aa signal sequence, an N-terminal VWA domain, four tandem EGF-like repeats, and a C-terminal coiled-coil domain (4). The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Human Matrilin-3 shares 82% aa sequence identity with mouse Matrilin-3. Within the first VWA domain, human Matrilin-3 shares approximately 55% aa sequence identity with human Matrilin-1, -2, and -4. The coiled-coil domain of Matrilin-3 mediates disulfide-linked homo-oligomerization, with tetramer formation being the most dominant (5-7). It can also assemble into hetero-oligomers with Matrilin-1 (5-7). Matrilin-3 is more plentiful than Matrilin-1 in the proliferative zone of the growth plate, whereas the reverse is true in the maturation zone (5). Matrilin-3 interacts directly with Collagen IX and COMP (8, 9). In the absence of Collagen IX, the expression of Matrilin-3 is unchanged, although it is retained inside chondrocytes and is not incorporated into the matrix (9). Matrilin-3 also associates with smaller cartilage fibrils independent of Collagen IX (9). Matrilin-3 knockout mice do not display any obvious abnormalities, suggesting that other molecules may compensate for the lack of Matrilin-3 (10). In contrast, intracellular retention of Matrilin-3 with particular point mutations in the VWA domain results in multiple epiphyseal dysplasia (11-13). A point mutation in the first EGF-like repeat which has been linked to hand osteoarthritis does not prevent Matrilin-3 secretion (13).

1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
2. Deak, F. et al. (1999) Matrix Biol. 18:55.
3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369. 
4. Belluoccio, D. et al. (1998) Genomics 53:391.
5. Zhang, Y. and Q. Chen (2000) J. Biol. Chem. 275:32628.
6. Klatt, A.R. et al. (2000) J. Biol. Chem. 275:3999.
7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
8. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
9. Budde, B. et al. (2005) Mol. Cell. Biol. 25:10465.
10. Ko, Y. et al. (2004) Mol. Cell. Biol. 24:1691.
11. Jackson, G.C. et al. (2004) J. Med. Genet. 41:52.
12. Cotterill, S.L. et al. (2005) Hum. Mutat. 26:557.
13. Otten, C. et al. (2005) J. Med. Genet. 42:774.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MATN3