MAP3K6 Antibody

Images

 
Western Blot: MAP3K6 Antibody [NBP1-51977] - (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

MAP3K6 Antibody Summary

Immunogen
Peptide with sequence C-RAILAQRAGSTP corresponding to C-Terminus according to NP_004663.3.
Epitope
C-RAILAQRAGSTP
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAP3K6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 140 kDa band observed in rat heart lysates (calculated MW of 143 kDa band according to human NP_004663.3 and rat NP_001101379.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-51977.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAP3K6 Antibody

  • apoptosis signal regulating kinase 2
  • ASK2MGC20114
  • EC 2.7.11
  • EC 2.7.11.25
  • MAPKKK6MEKK6Apoptosis signal-regulating kinase 2
  • MGC125653
  • mitogen-activated protein kinase kinase kinase 6

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP3K6 Antibody (NBP1-51977)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K6
Entrez
Uniprot