MAP3K6 Antibody

Western Blot: MAP3K6 Antibody [NBP1-51977] - (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MAP3K6 Antibody Summary

• Description: Novus Biologicals Goat MAP3K6 Antibody (NBP1-51977) is a polyclonal antibody validated for use in WB and ELISA. Anti-MAP3K6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RAILAQRAGSTP corresponding to C-Terminus according to NP_004663.3.
• Epitope: C-RAILAQRAGSTP
• Predicted Species: Human (100%), Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAP3K6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 140 kDa band observed in rat heart lysates (calculated MW of 143 kDa band according to human NP_004663.3 and rat NP_001101379.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAP3K6 Antibody

• apoptosis signal regulating kinase 2
• ASK2MGC20114
• EC 2.7.11
• EC 2.7.11.25
• MAPKKK6MEKK6Apoptosis signal-regulating kinase 2
• MGC125653
• mitogen-activated protein kinase kinase kinase 6

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP3K6 Antibody (NBP1-51977)(1)

Publication using NBP1-51977

• Eto N, Miyagishi M, Inagi R et al. Mitogen-Activated Protein 3 Kinase 6 Mediates Angiogenic and Tumorigenic Effects via Vascular Endothelial Growth Factor Expression. Am J Pathol 2009-04-01 [PMID: 19246638]

Bioinformatics

• Gene Symbol: MAP3K6
• Entrez:
  • 9064(Human)
  • 313022(Mouse)
  • 53608(Rat)
• Uniprot:
  • NP_004663.3(Human)