MAGED2 Overexpression Lysate (Native)

Images

 
Western Blot: MAGED2 Overexpression Lysate (Adult Normal) [NBL1-12816] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAGED2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAGED2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201748 and based on accession number NM_014599. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens melanoma antigen family D, 2 (MAGED2), transcript variant 1, mRNA.
Gene
MAGED2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
64.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MAGED2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAGED2 Overexpression Lysate (Native)

  • 11B6MAGE-D2 antigen
  • BCG-1
  • BCG1MAGEDHCA10
  • breast cancer associated gene 1
  • Breast cancer-associated gene 1 protein
  • hepatocellular carcinoma associated protein
  • hepatocellular carcinoma-associated protein HCA10
  • Hepatocellular carcinoma-associated protein JCL-1
  • JCL-1
  • MAGE-D2
  • melanoma antigen family D, 2
  • melanoma-associated antigen D2
  • MGC8386

Background

This gene is a member of the MAGED gene family. While the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). Multiple alternatively spliced transcript variants have been found for this gene, however, the full length nature of some variants has not been defined. Transcript Variant: This variant (1) encodes the same protein as variant 2 and variant 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-598
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
NB600-260
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-81162
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-00178
Species: Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
NB120-15160
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP1-89489
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54899
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
AF6604
Species: Hu
Applications: WB, IHC
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB38352
Species: Hu, Mu
Applications: WB, ICC
NB100-87021
Species: Hu
Applications: WB, IP, PLA
NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
AF567
Species: Mu, Rt
Applications: WB, Simple Western, IHC, Block
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-47386
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-48653
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-83549
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-46117
Species: Hu
Applications: WB, IHC, IF

Publications for MAGED2 Lysate (NBL1-12816) (0)

There are no publications for MAGED2 Lysate (NBL1-12816).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MAGED2 Lysate (NBL1-12816) (0)

There are no reviews for MAGED2 Lysate (NBL1-12816). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MAGED2 Lysate (NBL1-12816) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MAGED2 Products

Bioinformatics Tool for MAGED2 Lysate (NBL1-12816)

Discover related pathways, diseases and genes to MAGED2 Lysate (NBL1-12816). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MAGED2 Lysate (NBL1-12816)

Discover more about diseases related to MAGED2 Lysate (NBL1-12816).
 

Pathways for MAGED2 Lysate (NBL1-12816)

View related products by pathway.

Research Areas for MAGED2 Lysate (NBL1-12816)

Find related products by research area.

Blogs on MAGED2

There are no specific blogs for MAGED2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MAGED2 Antibody
NBP2-24694

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MAGED2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MAGED2