MAGE 1 Antibody (MA454) - Azide and BSA Free

Immunohistochemistry-Paraffin: MAGE 1 Antibody (MA454) - Azide and BSA Free [NBP2-33094] - Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu, Rt, Ca
• Applications: ELISA, IHC-P, S-ELISA
• Clone: MA454
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

MAGE 1 Antibody (MA454) - Azide and BSA Free Summary

• Immunogen: Recombinant full-length human MAGE 1 protein
• Localization: Cytoplasmic
• Specificity: Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-5ug/ml for coating
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in Sandwich -ELISA reported in scientific literature (PMID:31640756).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Alternate Names for MAGE 1 Antibody (MA454) - Azide and BSA Free

• Antigen MZ2-E
• Cancer/testis antigen 1.1
• cancer/testis antigen family 1, member 1
• CT1.1melanoma antigen family A 1
• MAGE-1 antigen
• MAGE1A
• MAGE1melanoma antigen MAGE-1
• melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• melanoma-associated antigen 1
• melanoma-associated antigen MZ2-E
• MGC9326

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA1