MAGE 1 Antibody (MA454)

Immunohistochemistry-Paraffin: MAGE 1 Antibody (MA454) [NBP2-29469] - Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu, Rt, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA
• Clone: MA454
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MAGE 1 Antibody (MA454) Summary

• Immunogen: Human MAGE-A1 full length recombinant protein.
• Localization: Cytoplasmic
• Specificity: Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
  • Sandwich ELISA
• Application Notes: Human Chromosome Location: Xq28
  Mol. Weight of Antigen: 42-46kDa
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. Use in Sandwich -ELISA reported in scientific literature (PMID:31640756).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for MAGE 1 Antibody (MA454)

• Antigen MZ2-E
• Cancer/testis antigen 1.1
• cancer/testis antigen family 1, member 1
• CT1.1melanoma antigen family A 1
• MAGE-1 antigen
• MAGE1A
• MAGE1melanoma antigen MAGE-1
• melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• melanoma-associated antigen 1
• melanoma-associated antigen MZ2-E
• MGC9326

Background

MAGE-1 is a cancer testis antigen (CTA) expressing in a variety of human cancers (Pereira CM et al., 2012). It is a human melanoma antigen recognized by cytolytic T lymphocytes. MAGE-1 induces a tumor specific immune response so it is thought to be a potential therapeutic target for cancer immunotherapy (Ma ZY et al., 2002). The gene for MAGE-1 belongs to a family of 12 genes known as MAGE-A cluster located on the X chromosome in region q28 (Laduron S et al., 2004). This relies primarily on DNA methylation for repression in somatic tissues but in many types of tumors, the promoter of these genes becomes demethylated and transcription becomes activated (Loriot A et al., 2006). Monoclonal antibody aganist a recombinant feline melanoma antigen 1 (fMAGE-1) has been generated and is effective against feline lymphoma cell lines and tumor tissues (Ma ZY et al., 2002). Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAGE 1 Antibody (NBP2-29469)(1)

Publication using NBP2-29469

• Mao Y, Fan W, Hu H et al. MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells J Hematol Oncol 2019 Oct 22 [PMID: 31640756] (S-ELISA)

Bioinformatics

• Gene Symbol: MAGEA1
• Uniprot:
  • P43355(Human)