Reactivity | Hu, Rt, CaSpecies Glossary |
Applications | WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, S-ELISA |
Clone | MA454 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Immunogen | Human MAGE-A1 full length recombinant protein. |
Localization | Cytoplasmic |
Specificity | Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | MAGEA1 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Human Chromosome Location: Xq28 Mol. Weight of Antigen: 42-46kDa Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. Use in Sandwich -ELISA reported in scientific literature (PMID:31640756). |
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Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Publications |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein G purified |
Publication using NBP2-29469 | Applications | Species |
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Mao Y, Fan W, Hu H et al. MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells J Hematol Oncol 2019 Oct 22 [PMID: 31640756] (S-ELISA) | S-ELISA |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-33094AF350 | |
Alexa Fluor 405 | NBP2-33094AF405 | |
Alexa Fluor 488 | NBP2-33094AF488 | |
Alexa Fluor 532 | NBP2-33094AF532 | |
Alexa Fluor 594 | NBP2-33094AF594 | |
Alexa Fluor 647 | NBP2-33094AF647 | |
Alexa Fluor 700 | NBP2-33094AF700 | |
Alexa Fluor 750 | NBP2-33094AF750 | |
Biotin | NBP2-33094B | |
DyLight 350 | NBP2-33094UV | |
DyLight 405 | NBP2-33094V | |
DyLight 488 | NBP2-33094G | |
DyLight 550 | NBP2-33094R | |
DyLight 594 | NBP2-33094DL594 | |
DyLight 650 | NBP2-33094C | |
DyLight 680 | NBP2-33094FR | |
DyLight 755 | NBP2-33094IR | |
FITC | NBP2-33094F | |
HRP | NBP2-33094H |
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