Recombinant Human MAF1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human MAF1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-256 of Human MAF1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSRGSGA EETSTMEEDR VPVICI

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MAF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human MAF1 Protein

  • DKFZp586G1123
  • homolog of yeast MAF1
  • MAF1 homolog (S. cerevisiae)
  • MGC20332
  • MGC31779
  • MGC39758
  • repressor of RNA polymerase III transcription MAF1 homolog

Background

MAF1 that is similar to Maf1, a Saccharomyces cerevisiae protein is a protein highly conserved in eukaryotic cells and is localized to the nucleus. Interactng with BRF2, MAF1 functions to mediate signals that specifically repress the activity of RNA polymerase III (Pol III), specifically by inhibiting the assembly of TFIIIB onto DNA. It responds to changes in the cellular environment and represses pol III transcription. Recombinant human MAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol MAF1