Recombinant Human MAF1 His Protein

SDS-Page: Recombinant Human MAF1 Protein [NBP1-72440] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MAF1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-256 of Human MAF1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSRGSGA EETSTMEEDR VPVICI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MAF1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MAF1 His Protein

• DKFZp586G1123
• homolog of yeast MAF1
• MAF1 homolog (S. cerevisiae)
• MGC20332
• MGC31779
• MGC39758
• repressor of RNA polymerase III transcription MAF1 homolog

Background

MAF1 that is similar to Maf1, a Saccharomyces cerevisiae protein is a protein highly conserved in eukaryotic cells and is localized to the nucleus. Interactng with BRF2, MAF1 functions to mediate signals that specifically repress the activity of RNA polymerase III (Pol III), specifically by inhibiting the assembly of TFIIIB onto DNA. It responds to changes in the cellular environment and represses pol III transcription. Recombinant human MAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MAF1