M-Ras/R-Ras3 Antibody (OTI3C4)

Western Blot: MRAS Antibody (3C4) [NBP2-00719] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRAS.

Immunocytochemistry/Immunofluorescence: MRAS Antibody (3C4) [NBP2-00719] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MRAS.

Flow Cytometry: MRAS Antibody (3C4) [NBP2-00719] - Analysis of Jurkat cells, using anti-MRAS antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.69 mg/ml

M-Ras/R-Ras3 Antibody (OTI3C4) Summary

• Immunogen: Full length human recombinant protein of human MRAS (NP_036351) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRAS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 23.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.69 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for M-Ras/R-Ras3 Antibody (OTI3C4)

• FLJ42964
• MRas
• M-Ras
• muscle and microspikes RAS
• muscle RAS oncogene homolog
• ras-related protein M-Ras
• Ras-related protein R-Ras3
• R-RAS3
• RRAS3M-RAs

Background

Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRAS
• Entrez:
  • 22808(Human)