Ly-6G6C Overexpression Lysate (Native)


Western Blot: Ly-6G6C Overexpression Lysate (Adult Normal) [NBP2-06220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6G6C.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

Ly-6G6C Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_079537. The protein contains a C-terminal DDK tag.
Homo sapiens Ly-6G6C, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Ly-6G6C

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ly-6G6C Overexpression Lysate (Native)

  • C6orf24
  • G6c
  • lymphocyte antigen 6 complex locus protein G6c
  • lymphocyte antigen 6 complex, locus G6C
  • NG24
  • UNQ1947/PRO4430


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Ly-6G6C Lysate (NBP2-06220). (Showing 1 - 1 of 1 FAQ).

  1.  I am running this lysate on a western as a positive control. Approximately what amount/volume is run on the gel?
    • Generally for our over-expression lysates, 10-25ug is recommended for loading on a gel as a positive control for the first time. For this particular lysate 10ug was tested first, and since a clear signal was seen, this image was used on the website. 

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Gene Symbol LY6G6C