LSM10 Knockout 293T Cell Lysate

Product Discontinued

LSM10 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,10 bp deletion in exon 2
• Gene: LSM10

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for LSM10 Knockout 293T Cell Lysate

• LSM10, U7 small nuclear RNA associated
• MGC15749
• MST074
• MSTP074
• U7 snRNA-associated Sm-like protein LSm10
• U7 snRNP-specific Sm-like protein LSM10

Background

Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Increases U7 snRNAlevels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cell cycle progressionfrom G1 to S phases. Binds specifically to U7 snRNA. Binds to the downstream cleavage product (DCP) of histonepre-mRNA in a U7 snRNP dependent manner

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LSM10