LPAR3/LPA3/EDG-7 Antibody

Immunocytochemistry/Immunofluorescence: LPAR3/LPA3/EDG-7 Antibody [NBP1-84903] - Staining of human cell line PC-3 shows localization to plasma membrane. Antibody staining is shown in green.

Product Discontinued

LPAR3/LPA3/EDG-7 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MNECHYDKHMDFFYNRSNTDTVDDWTGTK
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LPAR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: ICC/IF Fixation/Permeabilization: PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 26418031). Use in IHC-P reported in scientific literature (PMID:28739650).
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28739650).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LPAR3/LPA3/EDG-7 Antibody

• EDG7
• Edg-7
• EDG7FLJ98231
• GPCR
• HOFNH30
• LPA-3
• LPA3LPA receptor 3
• LPAR3
• lysophosphatidic acid G-protein-coupled receptor7,LPA receptor EDG7
• lysophosphatidic acid receptor 3
• Lysophosphatidic acid receptor Edg-7
• RP4-678I3

Background

EDG7, a Lysophospholipid/Lysosphingolipid Receptor, was identified by RT-PCR from Jurkat T-cells. It is activated by unsaturated lysophosphatidic acid LPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LPAR3/LPA3/EDG-7 Antibody (NBP1-84903)(2)

Publications using NBP1-84903

• Zhang MZ, Wang X, Yang H et al. Lysophosphatidic Acid Receptor Antagonism Protects against Diabetic Nephropathy in a Type 2 Diabetic Model J Am Soc Nephrol. 2017-07-23 [PMID: 28739650] (WB, IHC-P, Mouse)
• Leve F, Peres-Moreira RJ, Binato R et al. LPA Induces Colon Cancer Cell Proliferation through a Cooperation between the ROCK and STAT-3 Pathways. PLoS One 2015-01-01 [PMID: 26418031] (WB, Human)

Bioinformatics

• Gene Symbol: LPAR3
• Entrez:
  • 23566(Human)
• Uniprot:
  • Q9UBY5(Human)