LOX Antibody

Western Blot: LOX Antibody [NB110-59729] - Detection of mouse LOX protein in twice extracted guanidine hydrochloride mouse tibia/fibula lysate with NB110-59729 (lane 1) and normal rabbit serum control (lane 2).

Immunohistochemistry: LOX Antibody [NB110-59729] - Staining of LOX in mouse spleen.

Product Discontinued

LOX Antibody Summary

• Immunogen: Two synthetic peptides (within residues 150-200 and 275-325) of the rat LOX protein. [UniProt# P16636]
• Localization: Cytosolic and nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOX
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:250
  • Western Blot 1:4000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 28668305).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for LOX Antibody

• AAT10
• Lysyl Oxidase/LOX
• lysyl oxidaseEC 1.4.3.13
• MGC105112
• protein-lysine 6-oxidase

Background

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX Antibody (NB110-59729)(14)

Publications using NB110-59729

• Chandrakumar S, Santiago Tierno I, Agarwal M et al. Mechanical regulation of retinal vascular inflammation and degeneration in diabetes Diabetes 2023-11-21 [PMID: 37986627] (IHC-Fr, Mouse)
• Chandrakumar S, Tierno IS, Agarwal M et al. Subendothelial matrix stiffening by lysyl oxidase enhances RAGE-mediated retinal endothelial activation in diabetes Diabetes 2023-04-14 [PMID: 37058096]
• Nguyen NH, Kim D, Roy S High Glucose Increases Binding of Lysyl Oxidase to Extracellular Matrix Proteins: Implications for Diabetic Retinopathy Invest. Ophthalmol. Vis. Sci. 2020-04-09 [PMID: 32340032] (WB, IP, Rat)
• Chiavari M, Ciotti GMP, Navarra P, Lisi L Decreased lysyl oxidase level protects against development of retinal vascular lesions in diabetic retinopathy Exp. Eye Res. 2019-05-06 [PMID: 31022398] (WB, Mouse)
• Song B, Kim D, Nguyen NH, Roy S. Inhibition of Diabetes-Induced Lysyl Oxidase Overexpression Prevents Retinal Vascular Lesions Associated With Diabetic Retinopathy Invest. Ophthalmol. Vis. Sci. 2018-12-03 [PMID: 30550614] (WB, Rat)
• El Hajj EC, El Hajj MC, Ninh VK et al. Detrimental role of lysyl oxidase in cardiac remodeling J. Mol. Cell. Cardiol. 2017-08-01 [PMID: 28668305] (Rabbit)
• Kim D, Mecham RP, Trackman PC, Roy S. Downregulation of Lysyl Oxidase Protects Retinal Endothelial Cells From High Glucose-Induced Apoptosis. Invest. Ophthalmol. Vis. Sci. 2017-05-01 [PMID: 28538980] (WB, Mouse)
• Kanazawa I, Tomita T, Miyazaki S et al. Bazedoxifene Ameliorates Homocysteine-Induced Apoptosis and Accumulation of Advanced Glycation End Products by Reducing Oxidative Stress in MC3T3-E1 Cells. Calcif. Tissue Int. 2016-11-10 [PMID: 27832315] (Mouse)
• Yang X, Scott HA, Monickaraj F et al. Basement membrane stiffening promotes retinal endothelial activation associated with diabetes. FASEB J. 2015-10-06 [PMID: 26443820]
  
  Details:
  High glucose regulation of lysyl oxidase in endothelial cells and its effect on retinal endothelial activation is explored through the use of diabetic mouse models and atomic force microscopy.
• Dudakova L, Liskova P, Trojek T et al. Changes in lysyl oxidase (LOX) distribution and its decreased activity in keratoconus corneas Exp Eye Res 2012-10-03 [PMID: 23041260] (IHC-Fr, ICC/IF, Human)
• Remus EW, O'Donnell RE Jr, Rafferty K et al. The role of lysyl oxidase family members in the stabilization of abdominal aortic aneurysms Am J Physiol Heart Circ Physiol 2012-10-01 [PMID: 22904155] (WB, Mouse)
• Voloshenyuk T, Landesman E, Khoutorova E et al. Induction of cardiac fibroblast lysyl oxidase by TGF-b1 requires PI3K/Akt, Smad3, and MAPK signaling Cytokine 2011-07-01 [PMID: 21498085] (WB, Rat)
• Contente S, Yeh TJ, Friedman RM. Tumor suppressive effect of lysyl oxidase proenzyme. Biochim Biophys Acta 2009-07-01 [PMID: 19410608]
• Jeay S et al. Lysyl oxidase inhibits ras-mediated transformation by preventing activation of NF-kappa B. Mol Cell Biol;23(7):2251-63. 2003-04-01 [PMID: 12640111] (WB, Human)

Review for LOX Antibody (NB110-59729) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Corey . WB Other 06/05/2013

Summary

• Application: Western Blot
• Sample Tested: RF/6A whole cell lysate of rhesus monkey
• Species: Other
• Lot: B-2

FAQs for LOX Antibody (NB110-59729). (Showing 1 - 2 of 2 FAQs).

1. Hi, I was wondering if you have any blocking peptides for NB100-59729, NB100-2527 and NB100-2530?
   • Unfortunately, we do not currently have a blocking peptide available for NB110-59729.
2. Do you have more information regarding the image you provide on your website for the use of the LOX antibody in IHC on mouse spleen?
   • Our IHC image for product NB110-59729 was performed on FFPE mouse spleen tissue. The antibody was used at a dilution of 1:100. Antigen retrieval was pH 6.0 citrate buffer.

Bioinformatics

• Gene Symbol: LOX
• Entrez:
  • 4015(Human)
  • 16948(Mouse)
  • 24914(Rat)
• Uniprot:
  • P16636(Rat)