LMP7/PSMB8 Antibody (JB54-32)

Images

 
Western Blot: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of Proteasome 20S LMP7 on U937 (1) and A431 (2) cell lysate using anti-Proteasome 20S LMP7 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Staining Proteasome 20S LMP7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of paraffin-embedded rat epididymis tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin.
Flow Cytometry: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of Daudi cells with Proteasome 20S LMP7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary ...read more
Immunocytochemistry/ Immunofluorescence: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Staining Proteasome 20S LMP7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of paraffin-embedded human kidney tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of paraffin-embedded human liver tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody (JB54-32) [NBP2-75554] - Analysis of paraffin-embedded mouse small intestine tissue using anti-Proteasome 20S LMP7 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JB54-32
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LMP7/PSMB8 Antibody (JB54-32) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within C-terminal Human LMP7/PSMB8 . (SwissProt: P28062 Human;SwissProt: P28063 Mouse;SwissProt: P28064 Rat)
Localization
Nucleus. Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PSMB8
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LMP7/PSMB8 Antibody (JB54-32)

  • ALDD
  • beta5i
  • D6S216E
  • EC 3.4.25.1
  • JMP
  • LMP7
  • LMP7MGC1491
  • Low molecular mass protein 7
  • low molecular weight protein 7
  • Macropain subunit C13
  • Multicatalytic endopeptidase complex subunit C13
  • NKJO
  • protease component C13
  • proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7)
  • proteasome catalytic subunit 3i
  • Proteasome Component C13
  • proteasome subunit beta 5i
  • proteasome subunit beta type-8
  • Proteasome subunit beta-5i
  • Proteasome Subunit Y2
  • proteasome-related gene 7
  • PSMB5i
  • PSMB5iD6S216
  • PSMB8
  • Really interesting new gene 10 protein
  • RING10
  • RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7)
  • Y2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMB8