LMP7/PSMB8 Antibody (1A5)

Western Blot: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 mouse mAb with DAB staining.

Western Blot: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Western blot analysis using PSMB8 mAb against human PSMB8 (AA: 1-272) recombinant protein. (Expected MW is 55.2 kDa)

Immunohistochemistry: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 mouse mAb with DAB staining.

ELISA: LMP7/PSMB8 Antibody (1A5) [NBP2-37570] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

LMP7/PSMB8 Antibody (1A5) Summary

• Immunogen: Purified recombinant fragment of human PSMB8 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMB8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for LMP7/PSMB8 Antibody (1A5)

• ALDD
• beta5i
• D6S216E
• EC 3.4.25.1
• JMP
• LMP7
• LMP7MGC1491
• Low molecular mass protein 7
• low molecular weight protein 7
• Macropain subunit C13
• Multicatalytic endopeptidase complex subunit C13
• NKJO
• protease component C13
• proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7)
• proteasome catalytic subunit 3i
• Proteasome Component C13
• proteasome subunit beta 5i
• proteasome subunit beta type-8
• Proteasome subunit beta-5i
• Proteasome Subunit Y2
• proteasome-related gene 7
• PSMB5i
• PSMB5iD6S216
• PSMB8
• Really interesting new gene 10 protein
• RING10
• RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7)
• Y2

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMB8