LMO1 Overexpression Lysate (Native)

Images

 
Western Blot: LMO1 Overexpression Lysate (Adult Normal) [NBL1-12566] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LMO1.

Product Details

Summary
Applications WB

Order Details

LMO1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210233 and based on accession number NM_002315. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens LIM domain only 1 (rhombotin 1) (LMO1), mRNA.
Gene
LMO1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for LMO1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LMO1 Overexpression Lysate (Native)

  • Cysteine-rich protein TTG-1
  • LIM domain only 1 (rhombotin 1)
  • LMO-1
  • RBTN1MGC116692
  • RHOM1LIM domain only protein 1
  • rhombotin-1
  • TTG1T-cell translocation protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-78626
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-01488
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-80306
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-48693
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84843
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-31362
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00008048-M03
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-19548
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56424
Species: Hu, Mu, Rt
Applications: WB
NB600-480
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB, ELISA, IHC, IHC-P
H00055885-M06
Species: Hu
Applications: WB, ELISA, ICC/IF
MAB7650
Species: Mu
Applications: WB, IHC
NBP1-82454
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00030012-M01
Species: Hu
Applications: WB, ELISA
AF2605
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-27188
Species: Hu, Po, Ca, Pm
Applications: WB, Simple Western
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-91906
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00029107-M08
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for LMO1 Lysate (NBL1-12566) (0)

There are no publications for LMO1 Lysate (NBL1-12566).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LMO1 Lysate (NBL1-12566) (0)

There are no reviews for LMO1 Lysate (NBL1-12566). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LMO1 Lysate (NBL1-12566) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional LMO1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for LMO1 Lysate (NBL1-12566)

Discover related pathways, diseases and genes to LMO1 Lysate (NBL1-12566). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LMO1 Lysate (NBL1-12566)

Discover more about diseases related to LMO1 Lysate (NBL1-12566).
 

Pathways for LMO1 Lysate (NBL1-12566)

View related products by pathway.

PTMs for LMO1 Lysate (NBL1-12566)

Learn more about PTMs related to LMO1 Lysate (NBL1-12566).
 

Research Areas for LMO1 Lysate (NBL1-12566)

Find related products by research area.

Blogs on LMO1

There are no specific blogs for LMO1, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol LMO1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-12566 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106
p19ARF Antibody
NB200-106
LMO1 Antibody (4F7)
H00004004-M01