LIMS2 Antibody

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Immunohistochemistry-Paraffin: LIMS2 Antibody [NBP1-52185] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

LIMS2 Antibody Summary

Immunogen
Peptide with sequence C-RWSNMSDALA corresponding to internal region according to NP_001129509.2, NP_001154875.1.
Epitope
C-RWSNMSDALA
Specificity
This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIMS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot detection limit 1:8000
  • Immunohistochemistry 0.5 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
Application Notes
Western blot: Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Spleen.
Publications
Read Publication using NBP1-52185.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for LIMS2 Antibody

  • FLJ10044
  • ILK-binding protein
  • LIM and senescent cell antigen-like domains 2
  • LIM and senescent cell antigen-like-containing domain protein 2
  • LIM-like protein 2
  • Particularly interesting new Cys-His protein 2
  • PINCH2
  • PINCH-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIMS2 Antibody (NBP1-52185)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIMS2
Entrez
Uniprot