LIMD1 Antibody (919330) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 919330
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

LIMD1 Antibody (919330) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human LIMD1
  Met1-Val143
  Accession # Q9UGP4
• Specificity: Detects human LIMD1 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIMD1 Antibody (919330) [Alexa Fluor® 350]

• LIM domain-containing protein 1
• LIM domains containing 1
• LIMD1

Background

Human LIM Domain-containing Protein 1 (LIMD1) is a ubiquitous tumor suppressor 72 kDa protein which has three LIM zinc-binding domains. Via its LIM domains LIMD1 interacts with TRAF6, SNAI2/SLUG  and SCRT1 (via SNAG domain) proteins. It also interacts with SQSTM1 and RB1, EIF4E, AGO1, AGO2, DCP2, DDX6, LATS1, LATS2, EGLN1/PHD2, EGLN2/PHD1 and EGLN3/PHD3 proteins. LIMD1 interacts with isoform 1 and isoform 3 of VHL and with SNAI1 proteins. LIMD1 localized predominantly in the cytoplasm but shuttles between cytoplasm and nucleus where it can be often detected: strong nuclear expression is indicative for a low-tumor grade and a better patient prognosis. Down-regulation  of LIMD1 is in the nuclei of neoplastic cells, is the indication for a poor prognosis of breast cancer. The correlation exists between LIMD1 and IRF4 in cell lines derived from lymphomas. In ulcerative epithelium it was found that LIMD1 has a reduced nuclear expression. LIMD1 is encoded at chromosome 3p21.3, a region which is deleted in many solid tumors and LIMD1 is down-regulated in the majority of human lung cancer samples. LIMD1 also is a regulator of both bone osetoclast and bone osteoblast development and function. Human LIMD1 shares 77% aa sequence identity with both rat and mouse LIMD1.         

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.