LIM kinase 2 Antibody

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321] - THP cells. 1:500 antibody in DiluObuffer. Apparent MW is 76 KDa.

Immunohistochemistry-Paraffin: LIM kinase 2 Antibody [NBP3-12321] - Mouse Liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

LIM kinase 2 Antibody Summary

• Description: Novus Biologicals Chicken LIM kinase 2 Antibody (NBP3-12321) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid region 570-640 on LIMK2 protein.
• Clonality: Polyclonal
• Host: Chicken
• Gene: LIMK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin 1:50-1:150
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LIM kinase 2 Antibody

• EC 2.7.11.1
• LIM domain kinase 2
• LIM kinase 2
• LIMK2
• LIMK-2

Background

LIM-kinase 2 (LIMK2), a 75-kDa member of the LIM kinase family, is responsible for regulating actin polymerization via phosphorylation and inactivation of the actin depolymerizing factors ADF/cofilin (1). LIMK2 protein is found in the cytoplasm during interphase, redistributed to the mitotic spindle and then to the spindle midzone during anaphase to telophase (2). It has been shown that ROCK, a downstream protein kinase of Rho, activates LIMK2 by directly phosphorylates threonine 505 residue. Since activity of LIMK2 is cell cycle dependant, LIMK2 activity is markedly increased when HeLa cells are synchronized at mitosis with nocodazole treatment (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIMK2