Lgr4/GPR48 Antibody (OTI2D1)

Flow Cytometry: GPR48 Antibody (2D1) [NBP2-00974] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GPR48 antibody, and then analyzed by flow cytometry.

Western Blot: GPR48 Antibody (2D1) [NBP2-00974]-Analysis of overexpressedlysates (15ug per lane) from HEK293T cellstransfected with empty plasmid ([PS100001], NC),human LGR4 plasmid , (hLGR4),mouse LGR4 plasmid , mLGR4), ratLGR4 plasmid , rLGR4) using antiLGR4 antibody (1:5000@1mg/ml).Figure B, Western blot analysis of the samesamples as figure A with anti-DDK antibody 1:10000@1mg/ml)

• Reactivity: Hu
• Applications: WB, Flow
• Clone: OTI2D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Lgr4/GPR48 Antibody (OTI2D1) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 (NP_060960 ) produced in HEK293T Cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lgr4/GPR48 Antibody (OTI2D1)

• GPR48
• GPR48G protein-coupled receptor 48
• G-protein coupled receptor 48
• leucine-rich repeat containing G protein-coupled receptor 4
• Lgr4

Background

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Lgr4/GPR48 Antibody (NBP2-00974). (Showing 1 - 2 of 2 FAQ).

1. Is ab119314 from Abcam the same as NBP2-00974 from Novus? Because of the amount difference of supplemented information, Example Tested applications (Flow Cyt ) I have doubt, so I need your confirmation to show that these two are identical products.
   • Both antibodies have the same immunogen (human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 [NP_060960] produced in HEK293T Ce) and clone number (2D1). I have just confirmed that NBP2-00974 is useful for Flow Cytometery in addition to WB application. This antibody works well at following dilutions: Flow Cytometry 1:100, Western Blot 1:500.
2. May we ask if the product is powder or liquid?
   • This antibody comes in a liquid format.

Bioinformatics

• Gene Symbol: LGR4
• Entrez:
  • 55366(Human)
• Uniprot:
  • Q9BXB1(Human)