Layilin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Layilin Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Layilin
  Ala22-Glu220
  Accession # NP_849156
• Specificity: Detects human Layilin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant mouse Layilin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LAYN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Layilin Antibody (Catalog # MAB26371)
  Standard: Recombinant Human Layilin (Catalog # 2536-LA)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Layilin Antibody [Biotin]

• FLJ30977
• FLJ31092
• Layilin
• LAYN
• LYAN

Background

Layilin (named after the L-A-Y-I-L-I six amino acid motif in the molecule’s transmembrane segment) is a 55 kDa member of the animal C-type lectin family. The human Layilin cDNA encodes a 374 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 201 aa extracellular region, a 31 aa transmembrane segment, and a 123 aa cytoplasmic tail (1, 2). The extracellular domain has a 130 aa carbohydrate recognition domain that contains only one potential Ca⁺⁺-binding site. The intracellular region contains three types of LH (Layilin homology) repeats. There are three 16‑18 aa LH1 repeats, 3 five aa (E-G-S-F/W-V) LH2 repeats, and 2 four aa (N-D/E-I-Y) LH3 repeats. Adjacent LH2-LH3 tandem arrays provide docking sites for talin, a cytoskeleton-membrane adaptor protein (3). The human Layilin extracellular region is 85% aa identical to the hamster Layilin extracellular region, and 82% aa identical to the extracellular region of a similar molecule identified in mouse. Layilin is known to be a receptor for hyaluronan (HA), a negatively charged linear high molecular weight polysaccharide composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine (1, 4, 5). HA is found in the extracellular matrix of most animal tissues and in body fluids. Thus, it may be that Layilin modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. Layilin binds specifically to HA but not to other glycosaminoglycans (4).

1. Strausberg, R. (2002) GenBank Accession # AAH25407.
2. Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
3. Rees, D.J.G. et al. (1990) Nature 347:685.
4. Bono, P. et al. (2001) Mol. Biol. Cell 12:891.
5. Goa, K.L. and P. Benfield (1994) Drugs 47:536.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAYN
• Uniprot:
  • NP_849156()