Layilin Antibody (328024) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) Layilin or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human/Mouse Layilin Monoclonal Antibody (Catalog # MAB2637) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: 328024
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Layilin Antibody (328024) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Layilin
  Ala22-Glu220
  Accession # NP_849156
• Specificity: Detects human and mouse Layilin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAYN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Layilin Antibody (328024) [Unconjugated]

• FLJ30977
• FLJ31092
• Layilin
• LAYN
• LYAN

Background

Layilin is a type I transmembrane protein that acts as a receptor for hyaluronan. It has an extracellular C-type lectin domain and a 123 aa cytoplasmic domain that interacts with the cytoskeleton. The amino acid sequence of recombinant human Layilin is 85%, 82%, 80%, 79% and 76% identical to that of hamster, canine, bovine, mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAYN
• Uniprot:
  • NP_849156()