Latrophilin 2/LPHN2 Antibody (1030202) [Unconjugated]

Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human Latrophilin 2/LPHN2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Latrophilin 2/LPHN2 Monoclonal Antibody (Catalog # MAB105881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Latrophilin 2/LPHN2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

HEK293 Human Cell Line transfected with Human LPHN2 was stained with Mouse Anti-Human LPHN2 Monoclonal Antibody (Catalog # MAB105881, filled histogram) or isotype control antibody (MAB10041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 1030202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Latrophilin 2/LPHN2 Antibody (1030202) [Unconjugated] Summary

• Immunogen: Human Latrophilin 2/LPHN2 synthetic peptide
  Accession # O95490
• Specificity: Detects human Latrophilin 2/LPHN2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Latrophilin 2/LPHN2 Antibody (1030202) [Unconjugated]

• ADGRL2
• Calcium-independent alpha-latrotoxin receptor 2
• CIRL2
• CIRL-2
• CL2
• KIAA0786
• Latrophilin 2
• Latrophilin homolog 1
• latrophilin homolog 2 (cow)
• latrophilin-2
• LEC1
• LEC1LPHH1latrophilin 1
• lectomedin-1
• LPHH1
• LPHN2

Background

Latrophilin 2 (LPHN2) is a G-protein coupled receptor which functions in both cell adhesion and signal transduction. Latrophilin represents a phylogenetically conserved family of receptors which has an unusual structure consisting of two fragments that are produced by proteolytic cleavage of the parental molecule and behave independently in the plasma membrane. LPHN2 is a ubiquitous receptor for alpha-latroxin which is a protein toxin in black widow spider venom that is toxic to vertebrates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Latrophilin 2/LPHN2 Antibody (MAB105881)(1)

Publication using MAB105881

• A Timpanaro, C Piccand, AC Uldry, PK Bode, D Dzhumashev, R Sala, M Heller, J Rössler, M Bernasconi Surfaceome Profiling of Cell Lines and Patient-Derived Xenografts Confirm FGFR4, NCAM1, CD276, and Highlight AGRL2, JAM3, and L1CAM as Surface Targets for Rhabdomyosarcoma International Journal of Molecular Sciences, 2023-01-30;24(3):. 2023-01-30 [PMID: 36768928] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • O95490()