LAPTM4B Antibody (CL5289) - Azide and BSA Free

Analysis in human heart muscle and tonsil tissues using NBP3-44247 antibody. Corresponding LAPTM4B RNA-seq data are presented for the same tissues.

Staining of A-431 cells using the Anti-LAPTM4B monoclonal antibody) .

Staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.

Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.

Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Analysis in human cell line HEL.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL5289
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

LAPTM4B Antibody (CL5289) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse LAPTM4B Antibody (CL5289) - Azide and BSA Free (NBP2-61158) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q86VI4, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAPTM4B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for LAPTM4B Antibody (CL5289) - Azide and BSA Free

• LAPTM4beta
• LC27
• lysosomal associated protein transmembrane 4 beta
• lysosomal protein transmembrane 4 beta
• lysosomal-associated transmembrane protein 4B
• Lysosome-associated transmembrane protein 4-beta

Background

LAPTM4B( AAH14129, 1 a.a. - 227 a.a.) full-length recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAPTM4B