Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

Analysis in human duodenum and pancreas tissues using NBP3-43800 antibody. Corresponding Laminin beta 3 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows strong positivity in basement membrane in glandular cells.

Staining of human duodenum shows moderate positivity in basement membrane in glandular cells.

Staining of human prostate shows moderate positivity in basement membrane in glandular cells.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3363
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin beta 3 Antibody (CL3363) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Laminin beta 3 Antibody (CL3363) - Azide and BSA Free (NBP2-46623) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q13751, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin beta 3 Antibody (CL3363) - Azide and BSA Free

• BM600-125kDa
• Epiligrin subunit bata
• FLJ99565
• Kalinin B1 chain
• Kalinin subunit beta
• kalinin-140kDa
• LAM5
• Laminin 5
• Laminin B1k chain
• laminin S B3 chain
• laminin subunit beta-3
• laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
• laminin, beta 3
• Laminin-5 subunit beta
• LAMNB1
• Nicein subunit beta
• nicein-125kDa

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMB3